Control Flow Analysis for BioAmbients

This paper presents a static analysis for investigating properties of biological systems specified in BioAmbients. We exploit the control flow analysis to decode the bindings of variables induced by communications and to build a relation of the ambients that can interact with each other. We eventually apply our analysis to an example of gene regulation by positive feedback taken from the literature

Static Analysis for Systems Biology

This paper shows how static analysis techniques can help understanding biological systems. Based on a simple example will illustrate the outcome of performing three different analyses extracting information of increasing precision. We conclude by reporting on the potential impact and exploitation of these techniques in systems biology

Digital color-coded molecular barcoding reveals dysregulation of common FUS and FMRP targets in soma and neurites of ALS mutant motoneurons

Mutations in RNA binding proteins (RBPs) have been linked to the motor neuron disease amyotrophic lateral sclerosis (ALS). Extensive auto-regulation, cross-regulation, cooperation and competition mechanisms among RBPs are in place to ensure proper expression levels of common targets, often including other RBPs and their own transcripts. Moreover, several RBPs play a crucial role in the nervous system by localizing target RNAs in specific neuronal compartments. These include the RBPs FUS, FMRP, and HuD. ALS mutations in a given RBP are predicted to produce a broad impact on such delicate equilibrium. Here we studied the effects of the severe FUS-P525L mutation on common FUS and FMRP targets. Expression profiling by digital color-coded molecular barcoding in cell bodies and neurites of human iPSC-derived motor neurons revealed altered levels of transcripts involved in the cytoskeleton, neural projection and synapses. One of the common targets is HuD, which is upregulated because of the loss of FMRP binding to its 3'UTR due to mutant FUS competition. Notably, many genes are commonly altered upon FUS mutation or HuD overexpression, suggesting that a substantial part of the effects of mutant FUS on the motor neuron transcriptome could be due to HuD gain-of-function. Among altered transcripts, we also identified other common FUS and FMRP targets, namely MAP1B, PTEN, and AP2B1, that are upregulated upon loss of FMRP binding on their 3'UTR in FUS-P525L motor neurons. This work demonstrates that the impairment of FMRP function by mutant FUS might alter the expression of several genes, including new possible biomarkers and therapeutic targets for ALS

Juventudes e trabalho: trajetórias de egressos do programa Jovem Aprendiz

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas, Programa de Pós-Graduação em Psicologia, Florianópolis, 2015.O propósito do presente estudo foi compreender como se constroem as trajetórias de egressos do programa Jovem Aprendiz. Esta pesquisa integra as discussões feitas no Núcleo de Estudos Sobre Trabalho e Constituição do Sujeito (NETCOS) do Programa de Pós Graduação em Psicologia da UFSC, área dois -práticas culturais e processos de subjetivação e linha de pesquisa: processos de subjetivação, gênero e diversidades. As constantes transformações no campo do trabalho têm gerado mudanças objetivas e subjetivas na vida dos sujeitos que trabalham. Neste cenário, os jovens substancialmente são os mais afetados, estando expostos ao desemprego e trabalhos precarizados. Esta pesquisa, a partir de uma abordagem qualitativa, tomou a categoria trabalho como central e compreendeu as juventudes como uma construção social e em sua pluralidade embasada nas contribuições da psicologia histórico-cultural. O foco central da pesquisa foi o trabalho, no entanto, outras dimensões da biografia dos jovens foram consideradas (família, vida escolar), pois, elas encontram-se interconectas e atuam nas diversas construções, possibilidades e escolhas dos jovens. O método da história oral foi utilizado para conhecer as histórias laborais dos jovens egressos. As entrevistas reflexivas foram o principal procedimento de busca de informações. Foram entrevistados quatro jovens com idades entre 16 e 25 anos. A fotografia como memória e uma produção textual sobre projetos de futuro também foram usadas junto com as entrevistas, como procedimentos auxiliares. O processo de análise se deu por meio da organização de quatro núcleos de significação: Trajetória Laboral e Educacional, Experiências como Jovem Aprendiz, Ser Egresso e Projetos. Estes quatro núcleos foram discutidos a partir da história de cada jovem e depois entrecruzados a partir de suas similaridades e contraposições. Os sentidos expressos por meio das falas, imagens, e da produção textual reafirmam que a esfera do trabalho permanece como central para os jovens. Evidencia-se a necessidade de pensar ações relacionadas à qualificação, inserção e permanência dos jovens no campo laboral. Os sentidos que os jovens constroem sobre suas trajetórias expressam a multiplicidade e pluralidade da vivência da condição juvenil e do trabalho e são o entrelaçamento de escolhas biográficas e das estruturas de oportunidades disponíveis.Abstract : The purpose of this study was to comprehend how the senses of the path of the recent graduates from the program Young Apprentice were constructed. This study is part of the discussions made in the Study Center About Work and Constitution of the Subject ( NETCOS ) of the Graduate Program in Psychology of UFSC , area two cultural - practices and subjectivity and line processes of research: subjective processes , gender and diversity. The constant transformations in the labor market have generated objective and subjective changes in the lives of the working subjects. In this scenario, young adults are substantially more affected, being exposed to unemployment and precarious working conditions. This research, by using a qualitative approach, took labor as the central category and understood young adults as a social construct, and its plurality grounded on the contributions of cultural-historical psychology. The main focus of the research was labor, however, other dimensions of the biography of the young adults were considered (family, school life), because, they are embedded and work in the diverse constructions, possibilities and choices young adults make. The oral history method was used to learn about the labor history of the graduates. The primary method for obtaining information was through the reflexive interviews. We interviewed four young people aged 16 to 25 years. Photography as memory and a textual production on future projects were also used along with the interviews, as auxiliary procedures. The process of analysis was made possible through the organization of four main cores: Labor and Educational Paths, Experiences as a Young Apprentice, Being a Graduate and Projects. These four cores were discussed from each young adult?s history and then combined according to their similarities and contrapositions. The senses expressed through the talks, images, and the textual production reaffirms that labor remains as apriority to young adults, making the need to think about actions related to the qualification, entry and permanence of young adults in the labor market evident. The senses that young adults construct about their paths express the multiplicity and plurality of the experience of labor and youth conditions and are correlated with the bio graphical choices and structures of available opportunities

Spray drying of lipid nanosystems (SLN and NLC) loaded with Syzygium aromaticum essential oil

[EN] A quality by design approach was used to investigate the influence of formulation composition and spray drying conditions on physicochemical properties of redispersable lipid based nanosystems loaded with Syzygium aromaticum essential oil. Four critical independent variables were studied: presence or absence of the liquid lipid oleic acid (0% - 1%), of the cationic surfactant CTAB (0% and 1%), inlet drying temperature (60 ºC -80 ºC), and ratio of the drying aids (ADJ) regarded to total formulation constituents weight (1:1 and 2:1). Resuls showed the production of spray dried redispersable lipid systems loaded with essential is feasible under very restrict conditions.The authors express their gratitute to São Paulo State Research Foundation (FAPESP) for the financial aid (Grants 2014/15905-1; 2011/10333-1), and to Brazilian National Council of Research and Development (CNPq) for the fellowship to the first author.Rosa, D.; Oliveira, W. (2018). Spray drying of lipid nanosystems (SLN and NLC) loaded with Syzygium aromaticum essential oil. En IDS 2018. 21st International Drying Symposium Proceedings. Editorial Universitat Politècnica de València. 895-902. https://doi.org/10.4995/IDS2018.2018.7554OCS89590

A new approach to managing Lessons Learned in PMBoK process groups: the Ballistic 2.0 Model

In any organization, dealing with lessons learned is a complex issue that involves people, processes and technologies. Although lessons learned processes are already well established in the project management community, the use of modern web technologies to support them is still in its infancy. This paper introduces a new model to manage lessons learned in PMBoK process groups. This model draws upon interdisciplinary literature, which embeds lessons learned processes, shared context and Web 2.0 service models. The model is supported by Web 2.0 technologies and centered in PMBoK process groups to allow a thorough overview of the project. An exploratory focus group was set up to validate the model qualitatively within a constructivist ontology and an interpretive epistemology. The adoption of this model can help academics and practitioners using PMBoK process groups to acquire a better understanding of managing lessons learned in projects

Angiotensin II or epinephrine hemodynamic and metabolic responses in the liver of L-NAME induced hypertension and spontaneous hypertensive rats

AIM To study hepatic vasoconstriction and glucose release induced by angiotensin (Ang) II or Epi in rats with pharmacological hypertension and spontaneously hypertensive rat (SHR). METHODS Isolated liver perfusion was performed following portal vein and vena cava cannulationAng. or epinephrine (Epi) was injected in bolus and portal pressure monitoredglucose release was measured in perfusate aliquots. RESULTS The portal hypertensive response (PHR) and the glucose release induced by Ang. of L-NAME were similar to normal rats (WIS). On the other hand, the PHR induced by Epi in L-NAME was higher whereas the glucose release was lower compared to WIS. Despite the similar glycogen content, glucose release induced by Ang. was lower in SHR compared to Wistar-Kyoto rats although both PHR and glucose release induced by Epi in were similar. CONCLUSION Ang. and Epi responses are altered in different ways in these hypertension models. Our results suggest that inhibition of NO production seems to be involved in the hepatic effects induced by Epi but not by Ang.the diminished glucose release induced by Ang. in SHR is not related to glycogen content.Fundacao de Amparo a Pesquisa do Estado de Sao PauloCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Univ Fed Sao Paulo, Dept Biochem, Expt Hepatol Lab, BR-04023900 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Biosci, Expt Hepatol Lab, BR-11015020 Santos, BrazilUniv Fed Sao Paulo, Dept Med, Expt Hepatol Lab, BR-04023900 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Biochem, Expt Hepatol Lab, BR-04023900 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Biosci, Expt Hepatol Lab, BR-11015020 Santos, BrazilUniv Fed Sao Paulo, Dept Med, Expt Hepatol Lab, BR-04023900 Sao Paulo, BrazilFAPESP: 2011/13974-8|CAPESWeb of Scienc

Design sustentável: A transformação de pneus em superfícies táteis.

The present study highlights part of a research done in the field of surfaces design which is inserted in the main area of inclusive fashion design and has the objective of finding materials to develop textures for people with visual difficulty. During the research of materials that would attend the requirements of the project and also be sustainable, an alternative printing in discarded tires rubber was developed in partnership with craftsmen from the city of Ipuaçu, SC. These craftsmen already produce furniture with used tires, which made possible to acquire the necessary knowledge for handling the rubber. As analysis result, the project unites sustainability and design in the generation of knowledge and improves the quality of life for visually impaired people or people with visual difficulty in a material that may be applied in a variety of objects that are used in people’s lives